Chemical Composition And Rheological Study Of Polysaccharide From Eggs Of Aplysia Dactylomela
Volume 5 - Issue 5, May 2022 Edition
[Download Full Paper]
Author(s)
Jao Manarivo Ulrich Anito, Fenoradosoa Taratra André, Delattre Cédric, Michaud Philippe, Kall Briant
Keywords
Polysaccharide, Aplysia, dactylomela, rheology
Abstract
Aplysia dactylomela is a sea hare having eggs with high potential as polysaccharide collected in the coastal sea of Madagascar (Vohemar). To determine the chemical compositions, rheological characteristics, colorimetric assays, HPAEC and viscometer, measurements were carried out. Crude polysaccharides (EPPOAD) were extracted from Aplysia dactylomela eggs by the aqueous solvent extraction method under neutral and alkaline conditions. The extraction yield was 20.9 % and 22.89 % under those both conditions respectively. The results showed 31.24 % total sugar, 27.51 % neutral sugar, 17.92 % uronic acid, 3.93 % protein and 38.93 % sulphate. It is composed of fucose, arabinose, glucosamine, galactose, galacturonic acid and glucuronic acid assigned in g/g of sample 0.022, 0.049, 0.048, 0.146, 0.020, 0.023, respectively. The result showed that the EPPOAD extract has a shear-thinning behavior and a recovery of the critical concentration (C*) at 17.42 g/L.
References
[1]. N. Karaki, C. Sebaaly, N. Chahine, T. Faour, A. Zinchenko, S. Rachid and H. Kanaan, « The antioxidant and anticoagulant activities of polysaccharides isolated from the brown algae Dictyopteris polypodioides growing on the Lebanese coast ». Journal of Applied Pharmaceutical Science, vol. 3, no. 02, pp 043-051, 2013.
[2]. W. Yang, D. Chen, Z. He, L. Zhou, Y. Cai, H. Mao, N. Gao, Z. Zuo, R. Yin, J. Zhao, « NMR characterization and anticoagulant activity of the oligosaccharides from the fucosylated glycosaminoglycan isolated from Holothuria coluber ». Carbohydrate Polymers. Vol. 233, 115844, 2020.
[3]. Y. Sun, Z. Liu, S. Song, B. Zhu, L. Zhao, J. Jiang, N. Liu, J. Wang, X. Chen, « Anti-inflammatory activity and structural identification of a sulfated polysaccharide CLGP4 from Caulerpa lentillifera ». International Journal Bioogical Macromoles. Vol. 146, pp 931-938, 2020.
[4]. Y. Yu, Q. Song, L. Huang, M. Shen, Q. Yu, Y. Chen, J. Xie, « Immunomodulatory activities of sulfated Cyclocarya paliurus polysaccharides with different degrees of substitution on mouse spleen lymphocytes. Journal Functional Foods ». Vol. 64, 103706, 2020.
[5]. R.O. Souza, S.J.C. Madeira, F.D.S. Chagas, L.A. Parreiras, G.R.C. Santos, A.M.S. Assreuy, A.O. Mour and M.G. Pereira., « Purified polysaccharides of Geoffroe aspinosa barks have anticoagulant and antithrombotic activities devoid of hemorrhagic risks ». Carbohydrate Polymers. Vol. 124, pp 208-21, 2015.
[6]. T.A. Fenoradosoa, C. Delattre, C. Laroche, A. Wadouachi, V. Dulong, L. Picon, P. Andriamadio, P. Michaud, « Highly sulphated galactan from Halymenia durvillei (Halymeniales, Rhodophyta), ared seaweed of Madagascar matine coasts ». International journal of biological macromolecules. Vol. 45, pp 140-145, 2009.
[7]. M.H. Rakotoarisoa, T.A. Fenoradosoa, F. Abdoul, G. Pierre, J.H. Andriamadio, C. Delattre, P. Michaud and L. Clément, « Structural characterization and evaluation of biological activity of polysaccharides extracted from Haliptilon subulatum ». American Journal innovative research & applied sciences. Vol 13, no 1, pp 304-312, 2021.
[8]. A.V. Nogueira, G.R. Rossi, M. Iacomini, G.L. Sassaki, E.S. Trindade, T.R. Cipriani, « Viscera of fishes as raw material for extraction of glycosaminoglycans of pharmacological interest ». International Journal Biological Macromoles. Vol. 121, pp 239-248, 2019.
[9]. L. Chahed, R. Balti, S. Elhiss, N. Bouchemal, N. Ajzenberg, V. Ollivier, F. Chaubet, R.M. Maaroufi, M.B. Mansour, « Anticoagulant activity of fucosylated chondroitin sulfate isolated from Cucumaria syracusana. Process Biochemistry ». Vol. 91, pp 149-157, 2020.
[10]. J.H. Ryu, J. Sung, C. Xie, M.-K. Shin, C.-W. Kim, N.-G. Kim, Y.J. Choi, B.D. Choi, S.S. Kang, D. Kang, « Aplysia kurodai-derived glycosaminoglycans increase the phagocytic ability of macrophages via the activation of AMP-activated, protein kinase and, cytoskeletal reorganization in RAW264.7 cells ». Journal of Functional Foods. Vol. 27, pp 122-130, 2016.
[11]. M. Dhahri, S. Sioud, R. Dridi, M. Hassine, N.A. Boughattas, F. Almulhim, Z. Al Talla, M. Jaremko, A.M. Emwas, « Extraction, characterization, and anticoagulant activity of a sulfated polysaccharide from Bursatella leachii Viscera ». ACS Omega, Vol. 5, no 24, pp 14786-14795, 2020.
[12]. H. Matthews-Cascon, C.A. Rocha-Barreira, C.A.O. Meirelles, « Egg masses of some Brazilian mollusk ». Fortaleza : Expressão Gráfica e Editora. 119 p, 2011.
[13]. D.M. Pereira, P. Valentao, N. Teixeira, P.B. Andrade, « Amino acids, fatty acids and sterols profile of some marine organisms from Portuguese waters ». Food Chemistry. Vol. 141, no 3, pp 2412-2417, 2013.
[14]. T. Braga, M.J. Rodrigues, H. Pereira, J. Varela, L. Barreira, M. González-Wangüemert, L. Custódio, « Bursatella leachiifrom Mar Menor as a source of bioactive molecules : preliminary evaluation of the nutritional profile, in vitro biological activities, and fatty acids contents ». Journal of Aquatic Food Product Technology. Vol. 26, no 10, pp 1337-1350, 2017.
[15]. Q. Pan, Y. Sun, X. Li, B. Zeng, D. Chen, « Extraction, structural characterization, and antioxydant and immunomodulatory activities of a polyasaccharide from Notarchus leachii freeri eggs ». Bioorganic Chemistry. Vol. 116, 105275, 2021.
[16]. M. Dubois, K.A. Gilles, J.K. Hamilton, P.A. Rebers, F. Smith, « Colorimetric method for determination of sugars and related substances ». Analytical Chemistry. Vol. 28, no 3, pp 350-356, 1956.
[17]. M. Monsigny, C. Petit, and A.-C Roche, « Colorimetric determination of neutral sugars by a resorcinol sulfuric acid micromethod ». Analytical Biochemistry, Vol. 175, no 2. pp 525-530, 1988.
[18]. N. Blumenkrantz and G. Asboe-Hansen, « New method for quantitative determination of uronic acids ». Analytical Biochemistry. Vol. 54, no 2, pp 484-489, 1973.
[19]. K.S. Dodgson and R.G. Price, « A note on the determination of the ester sulphate content of the sulphated polysaccharide ». Biochimical journal. Vol. 84, pp 106-110, 1962.
[20]. O.H. Lowry, N.J. Rosenbrough, A.L. Farr, R.J. Randall, « Protein measurement with the Folin phenol reagent. Jounal biology chemistry ». Vol. 193, pp 265-275, 1951.
[21]. J.L. Funderburgh, « Keratan sulfate : structure, biosynthesis, and function ». Glycobiology. Vol. 10, no 10, pp. 951-958, 2000.
[22]. T.C.T. Lo, Y.H. Jiang, A.L.J. Chao, C.A. Chang, « Use of statistical methods to find the polysaccharide structural characteristics and the relationships between monosaccharide composition ratio and macrophage stimulatory activity of regionally different strains of Lentinula edodes ». Analytica Chimica Acta. Vol. 584, no 1, pp 50-56, 2007.
[23]. T.G. Mezger, « The rheology handbook : for users of rotational and oscillatory rheometers (2., rev. ed.) ». Hannover :Vincentz Network, 34 p, 2006.
[24]. R.P. Heldman and D.R. Singh, « Introduction to food engineering (5thed.) ». Amsterdam : Elsevier, 160 p, 2013
[25]. L. Utracki, and R. Shima, « Corresponding state relations for the viscosity of moderately concentrated polymer solutions ». Journal of Polymer Science. Vol. 1, pp 1089-1098, 1963
[26]. B. Petera, C. Delattre, G. Pierre, C. Vial, P. Michaud, T. A. Fenoradosoa, « Rheological Study And Prebiotic Potential Of Cereus Triangularis Cladodes Extract ». International Journal of Advanced Research and Publications. Vol. 4, no 3, pp 12-22, 2020.
[27]. R.J. Redgwell, C. Schmitt, M. Beaulieu, D. Curti, « Hydrocolloids from coffe : physicochemical and functional properties of an arabinogalactan-protein fraction from green beans ». Food Hydrocolloids. Vo. 19, pp 1005-1015, 2005.