Paper Details: Physiological Responses Of Rabbits To Oral Vitamin E Supplementation During Oxidative Stress Induced By Short-Term Hypothermia
Volume 3 - Issue 9, September 2019 Edition
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Johnson, N. C., Diri, M., Owen, O. J.
hypothermia, oxidative stress, rabbit and vitamin E
Physiological responses of rabbits to oral vitamin E supplementation during oxidative stress induced by short-term hypothermia were studied. Twenty four (24) male New Zealand White rabbits of 8 – 10 weeks of age and weighed 800-1000g were used in the experiment. The rabbits were randomly assigned to their treatment groups: T0 (control), T1 (hypothermia) and T2 (hypothermia + vitamin E). The experiment duration was six weeks. The animals were fed similar diets throughout the experimental duration except that T2 in the last four days of the experiment received 460mg/kg body weight of oral vitamin E (dl alpha tocopheryl acetate) supplementation daily. 24 hours after the last administration of vitamin E T0 rabbits were dipped into a water of body temperature (370C) whereas T1 and T2 rabbits were dipped into a water of 10-120C with their heads up for five minutes after which their blood samples were immediately collected and snap frozen. Body temperatures of the animals after dipping were T0 (37.9 ± 0.5), T1 (34 ± 1) and T2 (34.2 ± 0.7). Catalase (CAT) showed no significant differences (p > 0.05) in all the treatment groups. Superoxide dismutase (SOD) was significantly lower (p < 0.05) in T2 compared with T0 and T1 which had similar levels (p > 0.05). . Glutathione (GSH) was significantly different (p < 0.05) among treatments but T1 animals showed the lowest value. Glutathione Peroxidase (GSH-Px) was significantly different (p < 0.05) for all treatments but T1 animals showed the lowest concentration. Oxidized form of glutathione (GSSH) concentration was significantly different (p < 0.05) for all treatments but T2 animals demonstrated lowest concentration. Vitamin E concentration was significantly different (p < 0.05) among treatments with T2 animals showed the highest concentration. Malondialdehyde (MDA) concentration was significantly different (p < 0.05) for T0, T1 and T2 but T0 demonstrated the lowest value. It was concluded that administration of oral vitamin E at 460kg/mg of body weight can up-regulate the activities of anti-oxidants while simultaneously reduce pro-oxidants in rabbits during hypothermic condition.
(1) National academy of science. Recommended dietary allowance, 8th edition food and nutrition board, pp. 24-27. 1984
(2) I. M. Lee, N. R. Cork, J. M. Gaziamo, D. Gordon, P. M. Ridker, J. E. Manson, C. H. Hennekem, and J. E. Burring. Vitamin E in the primary prevention of cardiovascular disease and carncer: the women health study: A randomized control trial. JAMA 294 : 56- 65. 2005
(3) F. Lebas, P. Coudert, R. Rouvier, and H. DeRochambeair. The rabbit, husbandry, health and production. FAO Animal production and health series No 21, Food and Agricultural Organization, Rome. Italy. 1986
(4) J. Sephton, and J. Blake. Angora rabbits and their wool. Nimrod Press Ltd. Alton, Harits, UK. 1988
(5) A. Leyla and M. Ismail. Effect of oral vitamin E supplementation on oxidative stress in guinea pigs with short term hypothermia. Cell biochem.funct 25: 771-715. 2007
(6) K. Anderson, M. Simmons-menchaca, K. K. Lawson, J. Atkinson, B. G. Sanders, and K. Kline “Differential response of human and avian cancer cells to induction of apoptosis by vitamin E succinate and vitamin E analogue”, alpha-TEA. Cancer Res. 64: 4226-4269 2004
(7) S. Michael, and S. C. Navdeep. Reactive oxygen species in Redox signaling and oxidative stress. Curr.. bio. Journal ,Vol. 24 (10) : 882-895. 2010.
(8) E. S. Ford, U. A. Ajani, and A. H. Mokdad. Brief communication: the prevalence of high intake of vitamin E from the use of supplements among U. S. adults. AM intern. Med. 143: 116-120. 2005
(9) M. Alcala, M. Calderon-Dominguez, D. Serra, L. Herrero, M. P. Ramos, and M. Viana “Short-term vitamin E treatment impairs reactive oxygen species signaling required for adipose tissue expansion, resulting in fatty liver and insulin resistance to obese mice”. PLOS ONE 12 (10): journal.pone. 0186579. 2017
(10) H. Aebi. R. W. Sonja, S. Bernhard, and S. Frantisek “Heterogeneity of erythrocyte catalase” II. Vol. 48, issue 1. 1974. European journal of biochem
(11) H. Misra and I. Fridorich. The role of superoxide anion in the autoxidation of epinephrine and a simple assay for superoxide dismutase.journaal bio. Chem.. 247 (10): 3170-5175. 1972.
(12) S. Marklund, and G. Marklund. Involvement of the superoxide anion radical in the autoxidation of pyrogallorand a convenient assay for superoxide dismutase. European journal of biochem, 47(3): 469-474. 1974.
(13) N. Agergaard, and P. T. Jensen “Procedure for blood glutathione peroxidase determination in cattle and swine”. Acta vet. Scand. 23, 515-5271982
(14) I. B. Amara, N. Soudani, A. Troudi, H. Bouaziz, T. Boudawara, and N. Zeghal, “Antioxidant effect of vitamin E and selenium on hepatotoxicity induced by dimethoate in female adult rats”.Ecotex environ. Safe. Vol. 74:811-819, 2011
(15) A. B. H. Noor, A. H. Mohd, J. R. Rusdich, A. I. Ibrahim, S. B. Prasamit, M. Musalmah, A. M. Y. Yasmin, and Z. W. N. Wan. Effect of vitamin E on antioxidant enzymes and DNA damage in rats following eight weeks exercise. Nutritional journal 10:37. 2011.
(16) E. Devrim, H. Ozbek, and I. Durak I. ”Effects of high-cholesterol diet and antioxidant vitamins combination on oxidant/antioxidant status in heart, kidney, liver and testis tissues from rats”.Journal. Food lipids 14: 386-395. 2007
(17) R. J. Sokol. J. E. Heubi, S. Lannaccone, K. E. Bove, and W. F. Balistreri. Mechanism causing vitamin E deficiency during chronic childhood cholestasis. Gastroenterology 85, pp 1172-1182.1983
(18) S. A. Bucioli, C. A. Liuz, E. O. Vitor, L. Claudio and V. Helio“Effect of vitamin E supplementation on renal on renal non-enzymatic antioxidants in young rats submitted to exhaustive exercise stress”. J. of int. society for comp. med. Res. Vol. 11: 133 2011
(19) J. R. Michael, J. D. Holly, D. Megan, B. G. Kenneth, A. B. Brent, G. H.Gregory, G. C. Robert, and E. A. Stephen. Vitamin E and C supplementation reduces oxidative stress, improves antioxidant enzymes and positive muscle work in chronically loaded muscles of aged rats. ExpGerontol. 45 (11) : 882-895. 2010
(20) E. J. Gunde and C. Jorgen. Glutathione peroxidaseactivity of vitamin E and essential fatty acid deficient rats. Ann. Nutr. Metab. 25: 27-37. 1979
(21) S. Sabyasachi, C. Arijit, M. Chirandit, and K. C. Amer. Effect of vitamin E acetate supplementation on thyroid hormone-sensitive organs following exogenous L- thyroxine treatment. Asian journal of pharmacy and clinical research, vol. 11, issue 4, 2018.
(22) J. Kohrle, R. Brigelius-Flohe, A. Bock, R. Gartner, O. Meyer, and L. Flohe. Selenium in biology: facts and medical perspectives. Boil. Chem. 381: 849-864. 2000
(23) E. Mahaswari, G. R. Saraswathy, and T. Santhranii. Influence of vitamin E on hepatotoxicity and oxidative stress.Int. journal of research in pharmacy and biosciences. Vol. 2 (13) : 30-38 2015.
(24) S. Kalender, F. G. Uzun, D. Durak, F. Demir, and Y. Kalender. Malathion-induced hepatotoxicity in rats: effect of vitamin C and E. food chem. Toxicol. Vol. 48: 633-638. 2010
(25) NRC, Nutrient requirement of swine. 11th ed. Nat. acad. Press, 2012 Washington DC.
(26) R. Selvan, M. Saravanakumar, M. S. Suresh, G. Sureshbabu, M. Sasikumar, and D. Prashanth. Effect of vitamin E supplementation and high stocking density on the performance and stress parameters of broilers..brazilian journal of poultry science, vol. 9 (4):587-594.2017.
(27) SAS Institute, Inc. 1988. SAS/STAT User’s Guide, Release 6.03 edition; SAS Institute Inc. Cary, NC
(28) K. Ognik, and K. Wertelecki. Effect of different vitamin E sources and levels on selected oxidative stress indices in blood and tissues as well as on rearing performance of slaughter turkey hens. Journal of applied poultry research vol. 21: 259-271. 2012.