IJARP SJIF(2018): 4.908

International Journal of Advanced Research and Publications!

Laxative Potential Of Aqueous Extract Of The Aerial Parts Of Euphorbia Heterophylla In Loperamide-Induced Constipated Wistar Albino Rats

Volume 4 - Issue 1, January 2020 Edition
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Author(s)
Wellington, E.O, Peters, D.E, Onyeike, E.N
Keywords
Euphorbia heterophylla, loperamide, constipation, laxatives, faecal properties, electrolytes, wistar rats, gastrointestinal motility, intestinal fluid, biochemical assays
Abstract
Background and Objectives: Euphorbia heterophylla is a medicinal plant that is known to elicit several medicinal properties such anti-malaria, anti-anaemic, treatment of asthma and many other health problems. This study investigated the laxative potential of aqueous extract of the aerial parts of E heterophylla in constipated wistar rats. Materials and Methods: Constipation was induced by oral administration of 1ml of 3mg/kg body weight of loperamide for three days and the passage of dry and reduced number of faecal pellet was indicative of the episode of constipation in the rats. Results: The faecal properties such as number of faecal pellet, faecal, faecal water content and gastrointestinal motility were determine following the “Cho’’ method of determination faecal properties in condition of constipation. Plasma electrolytes (Na+, K+, Cl-, Ca2+ and Mg+) and urea concentrations were determined using colorimetric method. Significant increases (p≤0.05) were observed in the number of faecal pellet (171.29± 30.13), weight of faecal pellet (38.69± 2.92g) and faecal water content (2.01±0.12ml) in 1500mg/kg b.w treated group when compared to their respective control values. Gastrointestinal motility significantly increased (p≤0.05) for 1500mgkg b.w treated group (94.23%) in comparison to the constipated control (73.45%). Significant increase (p≤0.05) in concentrations of plasma sodium (Na+), potassium (K+), chloride (Cl-), magnesium (Mg2+), calcium (Ca2+) and urea (147.20 ± 1.48, 4.35± 0.09, 133.20 ± 2.05, 2.19 ± 0.01, 2.71 ± 0.01, and 9.25 ± 0.03 respectively) were observed for 1500mg/kg b.w treatment in comparison to the control values. Conclusion: The significant increases in the number of faecal pellet, weight of faecal pellet, faecal water content, gastrointestinal motility and the plasma electrolyte concentrations is indicative that aqueous extract of the aerial parts of Euphorbia heterophylla elicited laxative potential against constipation induction.
References
[1] Talley, N. J., Stanghellini V., Heading, R. C., Koch, K. L., Malagelada, J. R., and G.N.

[2] JTutgat, 1999. Functional gastroduodenal disorders. Gasterology, 45:1137–1142.

[3] Drossman, D. A., Li, Z., Andruzzi, E., Temple, R. D., Talley, N. J., and W. G. Thompson, (1999). Biochemical and Biophysical Models of Constipation and Diarrhea Caused by Incorrect Dose or Uneven Intake of Edible Oils (Fats). Central Gastroenterology, 5(8):347-4723.

[4] Rout, S.P., Choudhury, K.A., Kar, D.M., Das, L., A. Jain, 2003. Plants in traditional medicinal system-future source of new drugs. Internl. J. Pharmacy Pharmaceurical. Sci, 1(1):1-23.

[5] Mostafa, S. M., Bhandari, S., Ritchie, G., Gratton, N and R. Wenstone, 2003. Constipation and its implications in the critically ill patient. Br J Anaesth, 91(6):815-9.

[6] Kakino, M., Tazawa, S., Maruyama, H., Tsuruma, K., Araki, Y., Shimazawa, M and H. Hara, 2010. Laxative effects of agarwood on low-fiber diet-induced constipation in rats. BMC Complement Alternative Medicine, 15; 10 ():68.

[7] Rao, S. S and J. T. Go, 2010. Update on the management of constipation in the elderly: new treatment options. Clin Interv Aging, 9; 5():163-71.

[8] Okeniyi, S.O, Adedoyin, B.J., and S. Garba, 2012. Phytochemical screening, cytotoxicity, antioxidant, and antimicrobial activities of stem and leave extracts of Euphorbia heterophylla. Bull Environmental Pharmacology of Life Science, 1(8): 87-91.

[9] Unekwe, P.C., Ughachukwu, P.O., and J. O. Ogamba, 2006. Some pharmacological studies of aqueous extract of leaves of Euphorbia heterophylla. Tropical Journal of Medical Research, 10(2): 1-5.

[10] Falodun, A., Agbakwuru, E. O., and G. C. Ukoh, 2003. Antibacterial activity of Euphorbia heterophylla Linn (Family-Euphorbiaceae). Pakistan Journal of Scientific and Industrial Research, 4(3):31-42.

[11] Bustos, D., Ogawa, K., Pons, S., Soriano, E., Banji, J. C., and F. L. Bustos, 1991. Effect of loperamide and bisacodyl on intestinal transit time, fecal weight and short chain fatty acid excretion in the rat. Acta Gastroenterol Latin America, 21:3–9.

[12] Méité, S., Bahi, C and D. Yéo, 2010. Laxative activities of Mareya micrantha (Benth.) Müll Arg (Euphorbiaceae) leaf aqueous extract in rats. BMC Complement Altern Med, 10:7.

[13] Sagar, L., Sehgal, R and S. Ojha, 2005. Evaluation of antimotility effect of Lantana camara L. var. acuelata constituents on neostigmine induced gastrointestinal transit in mice. BMC Complement Altern Med, 5:18.

[14] Cazis, C. H., Timothy, S. Y., Zakama, S. G and B. Habiba, 2013.Phytochemical screening and purgative activity of ethanolic extracts of Vernonia amygdalina del. Leaf. International Journal of Research in Avurveda and Pharmacy, 4(1):46-49.

[15] Sabiu S., Omotayo, T., and A. Anofi, 2016. Toxicological implications and laxativepotential of ethanolic root extract of Morella serrata in loperamide-induced constipated Wistar rats. Pharmaceutical Biology, 54(12): 2901-2908.

[16] Ashafa, A. O.T, Sunmonu, T.O, and A. A. Abbass, 2011. Laxative potential of the ethanol leaf extract of Aleo vera (L.) in wistar rats. Journal of National Pharmacology, 2:158–162.

[17] Praveen, N., Nayak, S., Kar, D. M and P. Das, 2010. Pharmacological evaluation of ethanolic extracts of the plant Alternantherasessilis against temperature regulation. J. Pharm. Res., 3(6):1381-1383.

[18] Ahmad, I., Mehmood, Z and F .Mohammad, 1989. Screening of some Indian medical plant for their antimicrobial properties. J. Ethnopharmacon, 62(2):183-193.

[19] Apiamu, A., Evuen, U. F and U I. Ajaja, 2013.Biochemical Assessment of the Effect of Aqueous Leaf Extract Of Euphorbia Heterophylla Linn on Hepatocytes of Rats . IOSR Journal Of Environmental Science, Toxicology And Food Technology (IOSR-JESTFT) e-ISSN: 2319-2402,p- ISSN: 2319-2399. Volume 3, Issue 5.

[20] Yamada, K and Y. Onoda, 1993. Comparison of the effects of T-1815, yohimbine and naloxone on mouse colonic propulsion. Journal of Smooth Muscle Research, 29(2):47-53.

[21] Schiller, L. R., Santa, A. C. A., Morawski, S. G., and J.S Fordtan, 1984. Mechanism of the antidiarrheal effect of loperamide. Gastroenterology, 86:1475–1480.

[22] L. Degan , 2007. Gastrointestinal motility, physiology and methods of measurement. Gasterology, 64:195–199.

[23] Capasso, F., Mascolo, N., Autore, G and V. J. Romano, 1986. Laxative and production of antacids by colon of rat.. Pharmacological therapy, 38(8):627-9.

[24] Sabiu S., Omotayo, T., and A. Anofi, 2016. Toxicological implications and laxativepotential of ethanolic root extract of Morella serrata in loperamide-induced constipated Wistar rats. Pharmaceutical Biology, 54(12): 2901-2908.

[25] Ajani, E., Sabiu, S., Bamisye, F. A and B. A. Salau, 2014. Acute and sub-acute Toxicity Effects of Ethanolic Leaves Extract of Lagenaria brevifolia (Bitter gourd) on Hepatic and Renal Function of Rats . Journal of National Pharmacology, 2:158–162.

[26] Shahjahan, M., Sabitha, K. E., Mallika, J., and C. S. Shyamala, 2004. Effect of Solanum trilobattum against Carbon Tetrachloride induced hepatic damage in albino rats. Indian Journal of medical Research, 4, 194-198.

[27] Xi, Y., Laura, K., Schnackenberg, Q. S., and F. L. William, 2001. Hepatic biomarkers. Experimental and Clinical Science, 15:317-828.

[28] Pazhani, G. P., Subramanian, N. N., Arunchalam, G.., Hemalatha, S., and V. Ravichandran, 2001. Antidiarrheal potential of Elephantopus scaber Linn leaf extract. Indian drugs, 38 (5): 269-271.