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Comparative Cytotoxicity of Roasted Coffea Arabica And Leucaena Leucocephala Seed Extract Using Brine Shrimp Lethality Assay

Volume 6 - Issue 2, February 2023 Edition
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Coffee comprises compounds that contribute to the flavor and bioactivity of the brew. The complex reaction modifies the coffee’s chemical composition during roasting at high temperatures, with some beneficial compounds and some created. The Leucaena leucocephala is locally termed as “Ipil-ipil tree,”. Findings have revealed that it has antidiabetic and antioxidant properties. This study aims to analyze, determine and compare the level of cytotoxicity of Coffea arabica and Leucaena leucocephala using the brine shrimp lethality assay and validate the nutritional value, food edibility, and medical benefits of Leucaena leucocephala to be considered as one of the substitutes for coffee consumption. The general findings revealed that C. Arabica has (LC50) 10584.75 and L. leucocephala has (LC50) 1100.286, which indicates that both compounds have no toxicity. These findings are based on Meyers and Clarkson toxicity indexes. This study recommends that L. leucocephala seeds could be eaten and substituted for coffee, but consumption should be in moderation.
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