International Journal of Advanced Research and Publications (2456-9992)

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Isolation And Antibiogram Profile Of Multiple Drugs Resistance Pseudomonas Aeruginosa From Burn Wound Infection In Western Nepal

Volume 4 - Issue 1, January 2020 Edition
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Ram Bahadur Khadka, Balram Neupane, Bikram Khadka, Manoj Kafle
Antibiotic Susceptibility, Burn Infection, Multi Drug Resistance, Pseudomonas aeruginosa,
Different microorganisms that resist multiple antibiotics is considered to be most threatening problems to public health now a days. The aim of this study was to evaluate antibiotic resistance of Pseudomonas aeruginosa in burn wound infection and the antibiotic susceptibility pattern of P. aeruginosa isolates.Wound pus was collected using a sterile swab from all age group excluding the patients already on antibiotic therapy. One hundred eighty four (184) samples were collected from the wound pus in Medicross Diagnositc Center, Butwal, Nepal from October , 2017 through March , 2018.Out of 184 samples, 48 Pseudomonas aeruginosa strains were isolated and tested for antibiotic susceptibility against different antibiotics. The pattern of antibiotic susceptibility suggested that 100% of the isolates were resistant to Tobramycin, and 94.1% were resistance to Cefoperazone and Meropenem whereas the least resistance was shown against ciprofloxacin (35.3%) followed by amikacin and gentamicin (47.1%) both. The percentage of multi drug resistance in Pseudomonas aeruginosa isolates was 100% since all these positive samples were resistant to at least 3 drugs in the following classes: β-lactams, carbapenems, aminoglycosides and fluoroquinolones. In P. aeruginosa infections, periodic antimicrobial resistance monitoring is fundamental to update the current activity level of commonly used anti-pseudomonal drugs to minimize the risk of drug resistance.
[1] A.R. Lari, R. Alaghehbandan, Nosocomial infections in an Iranian burn care center, PubMed/11024608 Burn, 26 (2000) 737-40.
[2] I. Mohamed, F. Mohamed, V. Aslam, A. Hakeem, I. U. Rehman, A. Shah. “Wound infections and culture sensitivity pattern in pediatric burn patients, JPMI 23 4. (2009) 304-08.
[3] F.T.Ogunsola, O.Oduyebo, K.C. Iregbu, A.O. Coker, A.Adetunji, A review of nosocomial infections at LUTH; Problems and improvement Nigerian infection control association, J. Nigerian Infection Control Assoc. (1998) 1:14-20.
[4] J.LVincent., D.J. Bihari, P.M. Suter, The prevalence of nosocomial infection in intensive care units in Europe, J. Am Med Assoc. 274 (1995) 639–44.
[5] J.P. Pirnay, D. De Vos, C. Cochez, F. Bilocq, J. Pirson,M. Struelens, L. Duinslaeger, P. Cornelis, M. Zizi, A. Vanderkelen, Molecular Epidemiology of Pseudomonas aeruginosa Colonization in a Burn Unit: Persistence of a Multidrug-Resistant Clone and a Silver Sulfadiazine-Resistant Clone, J. clinical microbiology. (2003) 1192–1202.
[6] A. P. Fonseca, P. Correia, J. C.Sousa, R. Tenreiro, Association patterns of Pseudomonas aeruginosa clinical isolates as revealed by virulence traits,antibiotic resistance, serotype and genotype, FEMS Immunol Med Microbiol.51 (2007)505–16.
[7] P. R. Hsueh, L. J. Teng, P.C. Yang, Y. C. Chen, S.W. ho, K. T. Luh, Persistence of a Multidrug-Resistant Pseudomonas aeruginosa Clone in an Intensive Care Burn Unit, J. Clinical Microbiology. 36 (1998) 1347– 51.
[8] Cheesbrough, Monica. Medical Laboratory Manual for Tropical Countries: Vol.ii. Doddington (14 Bevills Close, Doddington, Cambs. PE15 0TT: M. Cheesbrough, 1984. Print.
[9] Dubey, R. C., and D. K. Maheshwari. Practical Microbiology. 2nd ed. N.p.: S.Chand and Comapany, 2006. Print. 160 - 205.
[10] Godkar, Dr.Praful B., and Dr.Darshan P. Godkar. Textbook of Medical Laboratory Technology. 2nd ed. N.p.: Bhalani House, Mumbai, 2003. Print.
[11] Panijarathinam, R. Practical Medical Microbiology. 1st ed. N.p.: Jaypee Brothers,Medical Pvt. Limited, 2008. Print. 1 - 192.
[12] CLSI: Clinical and Laboratory Standards Institute, Performance Standard for Antimicrobial Susceptibility testing; 21st Information Supplement .31 (2011) 1 – 165.
[13] N. F. Gang, Z. X. Zhuo, B. Jing, Z. Guoan, Large-area burns with pandrug-resistant Pseudomonas aeruginosa infection and respiratory failure, Chin Med J. 124: 3 (2011) 359-63.
[14] H. Salimi, P. Owlia, B. Yakhchali, A.R. Lari, Characterization of Pseudomonas aeruginosa in Burn Patients Using PCR- Restriction Fragment Length Polymorphism and Random Amplified Polymorphic DNA Analysis, Iran J. Med Sci. 35: 3(2010) 236-41.
[15] S.G. Pathmanathan, N.A. Samat, R. Mohamed, Antimicrobial susceptibility of clinical isolates of Pseudomonas aeruginosa from a Malaysian Hospital, Malaysian J. Medical Sciences. 16: 2 (2009) 27-3