Modified Protocol For Separation Of Chicks’ Intestinal Epithelium From Lamina Properia Applying Flow Cytometer
Volume 4 - Issue 2, February 2020 Edition
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Author(s)
Amgad, M. Abdelrahman, Osama A. Mohamed, Khalda A. Khalifa, Omar E. Abdelrahman, Dalia E. Maki, Azza B. Musa, Galal Eldin E. Mohamed, Amal, Mostafa
Keywords
Intestinal Epithelial Cells, salmonella enteritidis.
Abstract
Epithelial cells protect the surface of body cavities, and as they considered part of the immune system that fights invading bacteria. The epithelial barriers reduce the migration rate to host organs. Salmonella enteritidis is a facultative intracellular pathogen capable of surviving within non-phagocytes like epithelial cells and initiates the inflammatory responses. The current modified protocol was used for the separation of intestinal epithelial cells of newly hatched chicks from the submucosa and muscular layer that invaded by salmonella. Also, this protocol described the detaching of epithelial cells from its junction by using drop-shape steel lees stain loop and gently inverted the very small intestine of the chicks, then mechanical air pump through inverted tissue to separate epithelial cells, trypsin cells to ensure singlet status to be analysed by flow cytometer to study the invasion of microbial load and cytopathological status of epithelial cells.
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