Antioxidants And Oxidant Statuses Of Growing Pigs Fed Graded Levels Of Crude Oil-Contaminated Diets
Volume 4 - Issue 3, March 2020 Edition
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Author(s)
Johnson, N. C., Joy A. Okejim., J. A., Amakiri, A. O.
Keywords
Antioxidants, Crude Oil-Contaminated Diets, Oxidants and Pig.
Abstract
This study investigated the impacts of ingested graded levels of crude oil-contaminated diets on the antioxidant and oxidant statuses in the growing pig. 24 growing pigs weighing on average 8 ± 1.1 (mean ± SD) kg of body weight (BW) were used in the investigation. Pigs were randomly assigned to six dietary treatment groups of 4 pigs per treatment: 0g, 2g, 4g, 6g, 8g and 10g of crude oil/kg of diet, respectively. The experiment lasted for four weeks. Blood samples were collected from all treatment groups into EDTA treated tubes and immediately snap frozen for later anti-oxidant and oxidant analyses. Antioxidants: glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-P-x) and the oxidant malondialdehyde (MDA), respectively were analyzed for. There were no significant (P > 0.05) differences in all the antioxidants measured. Similarly, there were no significant (P > 0.05) differences in MDA contents for all dietary treatment groups. It was therefore concluded that crude oil contaminations up to 10g/kg of diet does not induce oxidative stress in growing pigs.
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