Vitamin E, Liver And Kidney Functions Of Rabbit During Hypothermia
Volume 4 - Issue 5, May 2020 Edition
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Author(s)
Ntinya C. Johnson , Moses Diri
Keywords
Vitamin E, liver and kidney functions, hypothermia and rabbit
Abstract
Effects of oral vitamin E supplementation on the health statuses of liver and kidney of rabbits during hypothermia were studied. 24 male New Zealand White rabbits of 8 – 10 weeks old and weighed about 800 – 1000g were used in the study. The rabbits were randomly assigned to three treatment groups as: T1 (control), T2 (hypothermia) and T3 (hypothermia + vitamin E). Experimental duration was 6 weeks. Animals were fed similar diets throughout the experimental period except that T3 animals received oral vitamin E daily at 460mg/kg body weight in the last 4 days of study. 24 hours after the last administration of vitamin E T1 rabbits were dipped into water of body temperature (370C) whereas T2 and T3 rabbits were dipped into water of 10 - 120C with their heads up for 5-minutes after which their blood samples were immediately collected and snap frozen. Body temperatures of the animals after dipping were T1 (37.9 ± 0.5), T2 (34 ± 1.0) and T3 (34.2 ± 0.7), respectively. Alanine amino transferase (ALT) serum concentrations were similar (P > 0.05) for T1 and T3 but T2 had significant higher (P < 0.05) ALT. Aspartic amino transferase (AST) was significantly higher (P < 0.05) in T2 compared with T1 and T3 that had similar (P > 0.0) AST values. Alkaline phosphatase (ALP) serum concentration was significantly different (P < 0.05) for T1, T2 and T3 with T2 showing the highest concentration. Blood urea nitrogen (BUN) levels were similar (P > 0.05) in T1 and T3 while T2 had a significant higher (P < 0.05) BUN value. Creatinine levels were similar (P > 0.05) in T1 and T3 while T2 had a significant (P < 0.05) higher value. It was concluded that vitamin E supplementation can up-regulate liver and kidney functions in rabbits during hypothermic condition.
References
[1]. Michael, S and Navdeep, S. C. 2014. Reactive oxygen species in Redox Signaling and
[2]. oxidative stress. J. Curr. Bio. 24 (10):R423 – R 453
[3]. 2. Alcala, M., Calderon-Dominguez, M., Serra, D., Herrero, L., Ramos, M. P., and Viana, M 2017. Short-term vitamin E treatment impairs reactive oxygen species signaling required foe adipose tissue expansion, resulting in fatty liver and insulin resistance to obese mice. PONE J. 12 (10): 35-43.
[4]. Hungu, C.W. Gathumbi, P. K. Maingi, N. and Nganga, C. J. 2013. Production characteristics and constraints of rabbit farming in central Nairobi and rift-valley province of Kenya. Livest. Res. Rural Dev. 25(1): 7-11.
[5]. Lalita, S. Amit, K. V. Anu, R. Amit, K. and Rajesh, N. 2016. Relationship between serum biomarkers and oxidative stress in dairy cattle and buffaloes with clinical mastitis. Biotechnol. 15 (3): 96-100.
[6]. Ekhato, C. N. Osifo, U. C. and Akpamu, U. 2014. Effect of oral contraceptive pills
[7]. (containing low doses of synthetic hormones) on liver function in adult female rabbits. Asian
[8]. J. Biotech. 6 (1): 15-20.
[9]. Kim. W. R. Flamm, S. L. Di Bisceglie, A. M. and Bodenheimer, H. C 2008. Serum activity of alanine amino transferase as an indicator of health and disease. Public policy committee of the American association for the study of liver disease. Hepatology 47 (4): 1363 – 1370.
[10]. Shokrzadeh, M. Shobi, S. Alfar, H. Shayegan, S. Payam, S. S. and Ghorbani, F. 2012. Effect of vitamin A, E, and C on liver enzymes activity in rat exposed to organophosphate pesticide (Diazinon). Pak. J. Biol. Sci.15 (19):936-941.
[11]. Zikic, R. V. Stajn, A.S. Pavlovic, S. Z. Ogndanovic, B. I. and Saicic, Z. S. 2011. Activities of superoxide dismutase and catalase in erythrocyte and plasma transaminases of gold fish exposed to cadmium. Physiol. Res. 50: 105-111.
[12]. Fulya, B. Fatih, M. Songul, C. Mustafa, O. Nuran, C. Y. and Sema T. O. 2012 Chemotherapeutic agent-inducedneprotoxicity in rabbits: protective role of grape seed extract. Int. J. Pharm. 8 (1): 39-45
[13]. Reitman, S. and Frankel, S. 1957. A colorimetric method for determination of serum glutamate oxaloacetate and glutamic pyruvate transaminase. Am. J. Clin. Pathol. 28: 56-58.
[14]. Aaron, B. 1930. Method of determining alkaline phosphatate in serum. J. Bio.
[15]. Chem. 89: 235- 247.
[16]. Machado, M. and Horizonte, B. 1958. Simple and rapid method of determination of urea by urease. Rev. Assoc. Med. Bras. 4:364-367.
[17]. Max J. 2011. Creatinine determination using picric acid in an alkaline environment. Oxford J. 4(2): 83-86
[18]. Arceci, R. J. Hann, I. M. and Smith, O. P. 2006. Paediatric haematology (3rd Ed.)
[19]. Wiley-Blackwell. Pp. 763-773
[20]. Aslan, L. and Meral, I. 2007. Effect of oral vitamin E supplementation on oxidative stress in guinea pigs with short term hypothermia. Cell Biochem. Funct. 25: 771-715.
[21]. Tapan, K. S. Veene, M. Harjik, K. Neelam, K. and Anjali, A. 2012. Effect of vitamin E supplementation on haematological and plasma biochemical parameters during long term exposure of arsenic in goats. Asian – Austra. J. Anim. Sci. 25(9):1262-1268.
[22]. Ognik, K., and Wertelecki (2012). Effect of different vitamin E sources and levels on selected oxidative stress indices in blood and tissues as well as on rearing performance of slaughter turkey hens. J. Appl. Poult. Res. 21: 259-271