Effects Of Feeding Graded Levels Of Crude Oil-Containing Diets On Liver And Kidney Functions In The Growing Pig
Volume 4 - Issue 5, May 2020 Edition
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Ntinya C. Johnson Joy A. Okejim , Annie, O. Amakiri
Liver and Kidney Biomarkers, Crude Oil, Contamination and Pig.
Effects of feeding graded levels of crude oil-containing diets were studied on liver enzymes: alanine amino transferase (ALT), aspartic amino transferase (AST) and alkaline phosphatase (ALP) as well as on kidney biomarkers: blood urea nitrogen (BUN) and creatinine in growing pigs. 24 pigs of average body weight (BW) of 9 Â± 1.4 (Mean Â± SD) kg were used in the study. Animals were randomly assigned to their individual experimental pens. There were 4 graded crude oil dietary treatments: 0g (control group), 10g, 15g and 20g of crude oil/kg of diet. There were 4 replications per dietary treatment. Animals were fed at 5% of their BW for 4 weeks. Blood samples were collected from all animal in all the four dietary treatment groups at the end and snap frozen. ALT serum levels of diets 1 and 2 were similar (P > 0.05) but significantly (P < 0.05) lower than those of diets 3 and 4 with diet 4 having the highest (P < 0.05) significant serum value of ALT. The trends of ALT were mimicked by AST and ALP, respectively. BUN serum levels of diets 1 and 2 were similar (P > 0.05) but significantly (P < 0.05) lower than those of diets 3 and 4 again with diet 4 showing the highest (P < 0.05) significant value. Creatinine mirrored BUN fashion. It was concluded that ingestion of 10g of crude oil/kg diet had no effect on the liver and kidney; however at 15g and above of crude oil/kg of diet the crude oil negatively affected the health of the liver and kidney based on increased sera levels of their biomarkers as yardsticks.
. Aaron, B. 1930. Method of determining alkaline phosphatate in serum. J. Bio. Chem. 89: 235-247.
. Ekhato, C. N. Osifo, U.C. and Akpamu, U. 2014. Effect of oral contraceptive pills (containing low doses of synthetic hormones) on liver function in adult female rabbits. Asian J. Biotech. 6(1): 15-20.
. Fulya, B., Fatih, M., Songul, C., Mustafa, O., Nuran, C.Y., and Sema T.O. 2012.
. Chemotherapeutic agent-induced nephrotoxicity in rabbits: protective role of grape seed extract. Int. J. Pharm. 8 (1): 39-45.
. Ganong, W. F. 2005. Review of Medical Physiology (23rd Ed.) Appleton and Large, Simo and Schister Coy, USA. Pp. 223-227.
. George, O. S. and Sese, B. T. 2012. Histopathological changes in the liver and kidney of adult buck rabbits exposed to crude oil contaminated feed. Global J. of Bio-science Biotechnol. 1(2):277-280.
. Ikanone, C. R. Akinloye, O. A. Ugbaja, R. N. Omotaiase, S. O. Ajayi, O. L. and Shopein, T. M. 2017. Effect of sub-acute exposure to Bonny Light crude oil on plasma and liver histopathology of Albino Rat. Anim. Res. Inter. 14(1):2652-2659.
. Johnson, N. C. Popoola, S. O. and Owen, O. J. 2019. Effects of single and combined antioxidant vitamins on growing pig performance and pork quality. Inter. J. Advance. Res. Public. 3(8):86-89.
. Kim. W. R., Flamm, S. L., Di Bisceglie, A. M., and Bodenheimer, H. C 2008. Serum activity of alanine amino transferase as an indicator of health and disease. Public policy committee of theAmerican association for the study of liver disease. Hepatology 47 (4): 1363 â€“ 1370
. Lalita, S. Amit, K.V. Anu, R. Amit, K. and Rajesh, N. 2016. Relationship between serum biomarkers and oxidative stress in dairy cattle and buffaloes with clinical mastitis. Biotechnol. 15 (3-4):96-100Machado, M. and Horizonte, B. 1958. Simple and rapid method of determination of urea by
. urease. Rev. Assoc. Med. Bras 4:364- 367 Max J. 2011. Creatinine determination using picric acid in an alkaline environment. Oxford J. 4(2): 83-86NRC, (2012). Nutrient Requirement of swine. 11th ed. Nat. Acad. Press, Washington DC.
. Ovuru, S. S. and Ekweozor, I. K. E. 2004. Haematological change associated with crude oil ingestion in experimentation rabbits. Afr. J. Biotech. 3(6):346-348.
. Shokrzadeh, M. Shobi, S. Alfar, H. Shayegan, S. Payam S. S. and Ghorbani, F. 2012.
. Effect of vitamin A, E, and C on liver enzymes activity in rat exposed to organophosphate
. pesticide (Diazinon). Pak. J. Biol. Sci. 15(19):936-941.
. Sumonu, T. O. and Oloyede, O. B. 2006. Changes in liver enzymes activities in African
. Catfish (Clarias gariepinus) exposed to crude oil. Asian Fishery Sci. 19:104-109.
. Zikic, R.V. Stajn, A. S. Pavlovic, S. Z Ogndanovic, B. I. and Saicic, Z. S. 2011. Activities of
. superoxide dismutase and catalase in erythrocyte and plasma transaminases of gold fish exposed to cadmium. Physiol. Res. 50: 105-111.
. Reitman, S. and Frankel, S. 1957. A colorimetric method for determination of serum glutamate oxaloacetate and glutamic pyruvate transaminase. Am. J. Clin. Pathol. 28: 56-58.