Histomorphological And Biochemical Evaluation Of Herbal Cocktail Used In Treating Malaria On Kidneys Of Adult Wistar Rats.
Volume 3 - Issue 7, July 2019 Edition
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Author(s)
Moronkeji A, Eze G.I, Igunbor M.C, Ogbonna A A, Moronkeji A.I
Keywords
Cocktail, Creatinine, Electrolytes, Malaria, Plasmodium berghei, Urea,
Abstract
Malaria continues to constitute a menace to Africa and the world at large and it remains a major problem in Nigeria with pregnant women and children mostly affected by this disease. This study investigates the histomorphology and biochemical activity of a herbal cocktail and its constituent extract on the kidneys of Plasmodium berghei infected Wistar rats. Thirty-five Wistar rats weighing an average of 200 g were divided into seven groups. Each group consisting of five rats were used in this study. The cocktail and aqueous extracts of Mangifera indica, Carica papaya, and Citrus limon were orally administered to the infected Wistar rats at a dose of 100 mg/kg/day for seven days while the aqueous leaf extract of Azadirachta indica was administered at a dose of 10 mg/kg/day. Blood was collected into anticoagulated bottles for biochemical assays for renal function tests while the kidneys were immediately excised and transferred into neutral buffered formalin for histological processing. Findings in this study showed that the aqueous extract of C. papaya and the cocktail were the most effective as no pathological lesions associated with malaria were observed in the kidneys, however extracts of M. indica was observed to be the least potent. Variations were observed in the electrolyte values obtained in different groups. Blood Urea values were observed to be normal among all groups with the exception of the group administered with aqueous leaf extract of C. papaya on comparison with the uninfected control group (p<0.03). Creatinine values were within the normal range among the various test groups on comparison with the uninfected control group (p>0.05). In conclusion, plants used in this study have potential antiplasmodial activities which could be exploited in malaria therapy.
References
[1]. BI. Nwiloh, AA. Uwakwe, JO. Akaninwor. “Biochemical effects of ethanolic extract from root bark of Salacia nitida L. benth in Plasmoduim berghei-malaria-infected miceâ€, American Journal of Physiology, Biochemistry and Pharmacology 9(1), pp. 1–8, 2019.
[2]. T. Ngarivhume, C.E.A. van’tKlooster, Joop T.V.M.deJong , Jan H.V. Westhuizen, “Medicinal plants used by traditional healers for the treatment of malaria in the Chipinge district in Zimbabweâ€, Journal of Ethnopharmacology 159 (2015), pp. 224–237, 2015.
[3]. MM. Taek, PEW. Bambang, A. Mangestuti. “Ethnomedicinal plants used for the treatment of malaria in Malaka, West Timorâ€. Journal of Young Pharmacists 10(2), 2018.
[4]. World Health Organization. World Malaria Report. Geneva. 2018. Licence: CC BY-NC-SA 3.0 IGO; ISBN 978-92-4-156565-3 available at https://creativecommons.org/licenses/by-nc-sa/3.0/igo
[5]. ML. Willcox, “A Clinical trial of ‘AM’, a Ugandan herbal remedy for malariaâ€, Journal of Public Health Medicine 21, pp. 318–324, 1999
[6]. M.L. Willcox, G. Bodeker. “Traditional herbal medicines for malaria. Clinical Reviewâ€, British Medical Journal 329, pp.1156–1159, 2004.
[7]. J.O. Adebayo, A.U. Krettli, “Potential antimalarials from Nigerian plants: A reviewâ€, Journal of Ethnopharmacology. 133, pp. 289–302, 2011.
[8]. BC. Akin-Osanaiye, AJ. Nok, S. Ibrahim, Inuwa HM, Onyike E, Amlabu E and E. Haruna. “Antimalarial effect of neem leaf and neem stem bark extracts on plasmodium berghei infected in the pathology and treatment of malariaâ€. International Journal of Research in Biochemistry and Biophysics 3(1), pp. 7-14, 2013.
[9]. Arzoo and Parina Kumari. “A review on plants having anti-malarial activityâ€, International Journal of Universal Pharmacy and Bio Sciences 6(3), pp. 142-158, 2017.
[10]. A. Moronkeji, GI. Eze, RA. Bejide, AA. Ogbonnaya and MC. Igunbor. “Evaluation of herbal cocktail used in the treatment of malaria on liver tissue of adult Wistar ratsâ€. Journal of Medicinal Plants Research.12(28), pp. 508-521, 2018.
[11]. Ascher, K. R. S. “Nonconventional insecticidal effects of pesticides available from the Neem tree, Azadirachta indicaâ€. Arch. Insect Biochem. Physiol. 22, pp. 433-449, 1993.
[12]. Schmutterer, H. “Properties and potential of natural pesticides from the Neem tree, Azadirachta indicaâ€. Ann. Rev. Entomol. 35, pp. 271-297, (1990).
[13]. A. Canini, D. Alesiani, D’Arcangelo G, Tagliatesta P. “Gas chromatography-mass spectrometry analysis of phenolic compounds from Carica papaya L. leafâ€. J Food Compost Anal 20, pp. 584–590, 1980.
[14]. Sadek KM. “Antioxidant and immunostimulant effect of Carica papaya linn. aqueous extract in acrylamide intoxicated ratsâ€. Acta Inform Med 20, pp.180–185, 2012.
[15]. A. Afzan, NR. Abdullah, SZ. Halim, BA. Rashid, RHR. Semail, Abdullah N, Jantan I, Muhammad H, Ismail Z. “Repeated dose 28-days oral toxicity study of Carica papaya L. leaf extract in Sprague Dawley ratsâ€. Molecules 17, pp.4326–4343, 2012.
[16]. G. Aravind, B. Debjit, S. Duraivel, G. Harish. “Traditional and medicinal uses of Carica papayaâ€. Journal of Medicinal Plants Studies 1, pp. 7–15, 2008.
[17]. TT. Nguyen, PN. Shaw, MO. Parat, AK. Hewavitharana. “Anticancer activity of Carica papaya: a reviewâ€, Mol Nutr Food Res 57, pp. 153–164, 2013.
[18]. P. Erasto, D. S. Grierson, and A. J. Afolayan “Bioactive sesquiterpene lactones from the leaves of Vernonia amygdalina,†Journal of Ethnopharmacology 106 (1), pp. 117–120, 2006.
[19]. WA. Gbolahan, AO, Titus, AA. Isiaka, EE. Gbolahan-Ayoade. “Composition of some traditional malaria remedies and their antiplasmodial effects on (Plasmodium berghei)â€. International Journal of Scientific Research Publications 4(3), pp. 1-8. 2014.
[20]. IT. Peter, VK Anatoli. The current global malaria situation. Malaria parasite biology, pathogenesis and protection. Washington D.C, USA: ASM Press pp. 11-12. 1998.
[21]. AE. Innocent, IP. Aniekan, NA. Aquaisua. “Histopathological effect of Nauclealatifolia ethanolic leaf extract and Artemether/Lumefantrine on the hippocampus of P. berghei-infected miceâ€. International Journal of Brain and Cognitive Sciences 6(1), pp. 9-16, 2017.
[22]. OG. Avwioro. “Histochemistry and Tissue Pathology; Principles and Techniquesâ€. 3rdEdition. Society for Cellular Pathology Scientist of Nigeria pp. 97-98, 2014.
