Physiological Responses Of Rabbits To Oral Vitamin E Supplementation During Oxidative Stress Induced By Short-Term Hypothermia
Volume 3 - Issue 9, September 2019 Edition
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Johnson, N. C., Diri, M., Owen, O. J.
hypothermia, oxidative stress, rabbit and vitamin E
Physiological responses of rabbits to oral vitamin E supplementation during oxidative stress induced by short-term hypothermia were studied. Twenty four (24) male New Zealand White rabbits of 8 – 10 weeks of age and weighed 800-1000g were used in the experiment. The rabbits were randomly assigned to their treatment groups: T0 (control), T1 (hypothermia) and T2 (hypothermia + vitamin E). The experiment duration was six weeks. The animals were fed similar diets throughout the experimental duration except that T2 in the last four days of the experiment received 460mg/kg body weight of oral vitamin E (dl alpha tocopheryl acetate) supplementation daily. 24 hours after the last administration of vitamin E T0 rabbits were dipped into a water of body temperature (370C) whereas T1 and T2 rabbits were dipped into a water of 10-120C with their heads up for five minutes after which their blood samples were immediately collected and snap frozen. Body temperatures of the animals after dipping were T0 (37.9 ± 0.5), T1 (34 ± 1) and T2 (34.2 ± 0.7). Catalase (CAT) showed no significant differences (p > 0.05) in all the treatment groups. Superoxide dismutase (SOD) was significantly lower (p < 0.05) in T2 compared with T0 and T1 which had similar levels (p > 0.05). . Glutathione (GSH) was significantly different (p < 0.05) among treatments but T1 animals showed the lowest value. Glutathione Peroxidase (GSH-Px) was significantly different (p < 0.05) for all treatments but T1 animals showed the lowest concentration. Oxidized form of glutathione (GSSH) concentration was significantly different (p < 0.05) for all treatments but T2 animals demonstrated lowest concentration. Vitamin E concentration was significantly different (p < 0.05) among treatments with T2 animals showed the highest concentration. Malondialdehyde (MDA) concentration was significantly different (p < 0.05) for T0, T1 and T2 but T0 demonstrated the lowest value. It was concluded that administration of oral vitamin E at 460kg/mg of body weight can up-regulate the activities of anti-oxidants while simultaneously reduce pro-oxidants in rabbits during hypothermic condition.
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