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Extraction, Utilization, Characterization And Confirmation Of The Structure Of Gorli Oil From The Dry Seeds Of The Traditional Medicinal Plant Caloncoba Echinata In Sierra Leone

Volume 2 - Issue 8, August 2018 Edition
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Author(s)
Lahai Koroma, T.B.R. Yormah, L.M. Kamara, G.M.T. Robert
Keywords
Antimicrobial activity, Soap production, Cosmetic production, saponification and iodine values, Epidermophyton floccosum, Candida albicans and dermatophytes.
Abstract
A novel source of non-edible vegetable oil (LKO3) for soap making and cosmetic production has been extracted from the dry seeds of Caloncoba echinata plant in Sierra Leone. 31.5% of fat was obtained from the dry weight of seeds of the plant much higher than palm oil obtained from the Palm tree fruits. Unlike other unsaturated fatty acids, LK03 is solid at room temperature having a fairly high melting point (68.5 °C) and boiling point (247.5 °C) with Saponification Value (89.76) and Iodine value (99.00) supporting the use of the oil in soap making and cosmetic production. Antimicrobial sensitivity tests carried out on the Oil (LK03) using bacterial and fungal isolates showed very potent antibacterial activity on Escherichia coli and Staphylococcus aureus known to stimulate wound and skin infections and antifungal activity on Epidermophyton floccosum and Candida albicans known to cause dermatophytes. These results support the use the oil extracted from the seeds of Caloncoba echinata in healing of wounds and skin eruptions in Traditional Medicine. The structure of LK03 was confirmed by both NMR400 Bruker Bio.Spin GmbH (Germany), LCMS/ GCMS analysis (China), by McLafferty Rearrangement and identified as d-13 (2-cyclopentenyl) tridecanoic acid.
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