Extraction And Determination Of Chlorophyll Content From Microalgae
Volume 1 - Issue 5, November 2017 Edition
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Author(s)
Yu Yu Nwe Oo , Myat Chaw Su , Kay Thi Kyaw
Keywords
Chlorella, Nannochloropsis, chlorophyll, spectrophotometric method, SCOR-UNESCO equation
Abstract
In this experiment, we selected the two microalgae species, Chlorella and Nannochloropsis for their numerous benefits. The microalgae was cultured with Conway medium under laboratory condition to get biomass for the further experiments. The concentration of chlorophyll a and b was evaluated by these procedures; extraction of chlorophyll by solvent, determination of chlorophyll content by spectrophotometric method and calculation by SCOR-UNESCO equation. So, two types of chlorophyll a and b are present in the green microalgae. According to the results, Chlorella has chlorophyll a (8.45 µg/ml) and Chlorophyll b (4.33 µg/ml), Nannochloropsis has chlorophyll a (21.24 µg/ml) and chlorophyll b (9.66 µg/ml) respectively.
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