Characterization Of Cellulolytic Multi-Enzymatic Complexes From Filamentous Fungi By Solid-State Fermentation Using Wheat Straw As Carbon Source
Volume 1 - Issue 5, November 2017 Edition
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Author(s)
Lydia Toscano, Margarita Stoytcheva, Gisela Montero, Lourdes Cervantes, Gerardo Medina, Rosa MarÃa Félix
Keywords
Cellulolytic enzyme, filamentous fungi, halostable and thermostable enzyme.
Abstract
The aim of the present study is to identify thermostable and halostable enzymes from filamentous fungal strains. Trichoderma harzianum, Aspergillus niger and Aspergillus flavus show the highest cellulolytic enzyme activity. The cellulolytic enzyme activities of these species were characterized using filter paper, carboxymethyl cellulose and microcrystalline cellulose. The optimum temperature range for the activity of endoglucanase, a component of the enzymatic complexes, is 65–70 °C, whereas the optimum range for exoglucanase is 55–70 °C. All the enzymes are thermostable at 40–70 °C. At a temperature of 60 °C, for all three species, the optimal pH for endoglucanase and exoglucanase activity is 6 and 5.5 respectively. The results of a saline stability test indicate that more than 80% of the enzyme activity is retained for 1 h in 25% sodium chloride. Using carboxymethyl cellulose as substrate, T. harzianum exhibits a Michaelis-Menten constant (Km) of 19.53 mg.mL-1 (the lowest) and maximum velocity (Vmax) of 0.4 µmol.min-1.mL-1.The high halostability and thermostability of the cellulases produced by these fungal strains point to their potential use in harsh industrial processing conditions.
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