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In-Vivo Antioxidant Potentials Of Citrullus Colocynthis Schard In Rotenone Induced Parkinson’s Disease Animal Model.

Volume 2 - Issue 11, November 2018 Edition
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Author(s)
Dr.Tausif Ahmed, Dr. Shivaprasad Hudeda
Keywords
Citrullus colocynthis, Antioxidant, Rotenone, Parkinson’s disease.
Abstract
the current study aims at investigating the Neuroprotective efficacy of an herb INDRAVARUNI – Citrullus colocynthis Schard by estimating its effect on endogenous antioxidant molecules in rat brain samples, using Rotenone induced Parkinson’s disease animal model. 42 healthy male Wistar rats were selected for the study and were divided into 7 groups. Before inducing Parkinson’s disease, the experimental animals were acclimatized for three days. The Rotenone solution was administered at 2.5mg/kg body weight/day intraperitonealy to all the groups, except for the normal control group which received only the vehicle (DMSO + Triester F 810). The respective treatment groups received their treatment from the day of starting of rotenone injection. Animals developing debilitating phenotype – limiting their mobility, feeding or grooming; were sacrificed and brain samples procured from them. Ultimately the rats surviving till the 9th day were also sacrificed on 10th day and brain samples procured, which were used for estimating MDA, SOD, CATALASE and GSH levels. The BID and TDS dosage groups showed maximum efficacy in endogenous antioxidant parameters.
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